Forum Topic: Cassava Transcriptome Research
I am a college student and work in plant breeding. I want to research genome-wide analysis of cassava disease-related gene families. Any suggestions?
This topic was started by Alex Steven.
Arabica coffee (approximately 70% of world coffee consumption) is one of the most important and valuable commodities in international trade1. The coffee bean is a tropical dicot protein seed with abundant endosperm and a small embryo. In general, seeds have evolved complex strategies to store nutrients and use the peel and pulp to encourage the spread of animals 2. The composition of coffee beans determines the quality of coffee. A better understanding of the molecular basis for determining the composition of coffee beans is needed to support increased coffee production and genetic improvement, especially in response to climate change. Learn more about coffee: https://roastercoffees.com/.
The composition of mature coffee beans determines the processing performance and final quality of coffee produced from coffee beans. The analysis of the differences in gene expression during coffee bean maturation can explain the basis of the genetic and environmental variation of coffee quality.
A large number of biochemical studies have been conducted on genetic and environmental factors that affect the accumulation of critical components of legumes. The genetic control of these processes can be studied by studying the changes in gene expression during bean maturation, including the regulation of bean filling and stress-responsive transcripts. Early research mainly applied RT-PCR (coffee beans) or microarrays to coffee leaves or seedlings. Recently, transcriptome analysis has been conducted on different tissues of Arabica coffee, including flowers, leaves, and peels. A recent genome-wide study reported the identification of critical genes that regulate the lipid and diterpene content of Arabica coffee beans. However, these studies did not include genetic analysis of other important components of coffee beans. Importantly, the absence of the reference genome or transcriptome further limited previous studies. In addition, there are still gaps in the knowledge of gene interactions and how the expression of specific maturation stages changes on a global scale and ultimately determines the quality and regenerative capacity of beans. Get more: https://roastercoffees.com/caffeine-calculator/
This study collected green, yellow, and red coffee beans at different developmental stages (except exocarp or mesocarp), and RNA was sequenced for transcriptome analysis. The recent Long Read Sequencing Full Length (LRS) coffee bean transcriptome was used as a reference to facilitate transcriptome analysis of mature coffee beans.
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